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2x multif seamless assembly mix kit  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology 2x multif seamless assembly mix kit
    2x Multif Seamless Assembly Mix Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multif+seamless+assembly+mix+kit/2x+multif+seamless+assembly+mix+kit/pmc12145288-48-30-36
    Average 90 stars, based on 1 article reviews
    2x multif seamless assembly mix kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: A yeast surface display platform for screening of non-enzymatic protein secretion in Kluyveromyces lactis.
    Article Snippet: To construct YSD strains with different anchors, four commonly used GPI-CWPs (α-agglutinin, Sed1, Cwp2, and C-terminal 428 amino acids of Flot1) encoded by S. cerevisiae genome were chosen (accession numbers in Table S3). .. Their coding sequences, excluding signal peptides, were amplified by PCR and assembled into the pKLAC1-IL-1β vector using the ABclonal MultiF Seamless Assembly Mix kit (ABclonal Inc., Wuhan, China). .. Unless explicitly stated, all constructs used the α-mating factor from the pKLAC1 vector as a signal peptide.

    Polymerase Chain Reaction:

    Article Title: A yeast surface display platform for screening of non-enzymatic protein secretion in Kluyveromyces lactis.
    Article Snippet: To construct YSD strains with different anchors, four commonly used GPI-CWPs (α-agglutinin, Sed1, Cwp2, and C-terminal 428 amino acids of Flot1) encoded by S. cerevisiae genome were chosen (accession numbers in Table S3). .. Their coding sequences, excluding signal peptides, were amplified by PCR and assembled into the pKLAC1-IL-1β vector using the ABclonal MultiF Seamless Assembly Mix kit (ABclonal Inc., Wuhan, China). .. Unless explicitly stated, all constructs used the α-mating factor from the pKLAC1 vector as a signal peptide.

    Article Title: Food-grade expression of multicopper oxidase with improved capability in degrading biogenic amines
    Article Snippet: .. Then, the purified PCR products of MCOs and pNZ8149 were assembled by the Gibson assembly method using the ABclonal MultiF Seamless Assembly Mix kit (ABclonal Technology, Wuhan, China) [43]. ..

    Mutagenesis:

    Article Title: A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeres
    Article Snippet: To make the GST-Scc1 (281–420)-SA2 (80–1060) constructs, the PCR fragments of Scc1 was inserted into the BamHI/SalI sites of pGEX-6P-2rbs (Amersham Pharmacia Biotech), then the PCR fragments of SA2 was inserted into the BglII/XhoI sites. .. All point mutations were introduced with the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) or by the MultiF Seamless Assembly mix kit (ABclonal Biotechnology). ..

    Purification:

    Article Title: Food-grade expression of multicopper oxidase with improved capability in degrading biogenic amines
    Article Snippet: .. Then, the purified PCR products of MCOs and pNZ8149 were assembled by the Gibson assembly method using the ABclonal MultiF Seamless Assembly Mix kit (ABclonal Technology, Wuhan, China) [43]. ..



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